The Compression settings were Graphics and Grayscale, and the keyframe every box was unchecked.
#MORPH AGE SAVING AS A TIFF MOVIE#
The movie was then exported and appended with (web).mov. mov extension, and the radio button that says make movie self-contained was checked. The images were used to make a master movie of PICT or TIFF files.
In Open Image Sequence, a frame rate of 15 frames per second was chosen. This was done by opening the TIFF images in Movieplayer Pro. Quicktime movies were also made from the images. After this, the new GIF images were renamed in File Buddy with the appendix GIF. This was done with a Batch Action in Photoshop. Next, the images needed to be put in GIF format for use on the Web. The images were then renamed in File Buddy: starting with COR000.PIC, SAG000.PIC, and HORI000.PIC. This was done for all three slice planes. The numbered files were then stacked, and saved to files as PICT images. After applying this macro, all the images were numbered. To get white numbers on the black background, the color value for the macro was changed from 255 to 0. The macro used to number the images was an NIH Editing macro called Draw File Name in Each Image. The renamed images were then all opened in NIH Image. The Basename was deleted, the Start numbering was set at 000, the Increment was kept at 1, and the Append to each name was left empty. The first step in numbering the images was to rename the TIFF images in File Buddy. This was done with a Photoshop Batch Action to a size of 320X320 pixels for the coronal plane, and 320 pixels width X154 pixels height for the sagittal and horizontal planes. The images then had to be reduced in size for the web. The images were then stacked in NIH Image, and resliced into the other two slice planes (sagittal and horizontal) after calculating the interslice spacing of 2.712582 pixels. The Input numbers used were 50,1,255, and the Output numbers were 0,239. This was done in Photoshop's Image Adjusting Levels window, and performed as a Batch Action on all the images. The first step in preparing the images for this site was getting the image contrast at an optimum level.
#MORPH AGE SAVING AS A TIFF SOFTWARE#
The animations and slices seen on this web site were processed using the following software programs: Adobe Photoshop 4.01PPC, NIH Image 1.62b17/PPC, File Buddy 5.01 PPC, and Movieplayer Pro. This can be corrected by running frequent recalibrations. In this dataset, the rings are very faint in the affected slices. In the original plane, which is coronal, there are concentric rings caused by detectors drifting off line. The Herrerasaurus dataset has ring artifacts. The interslice spacing and field of reconstruction were used with the image dimensions to get the interslice spacing in pixels needed to reslice the data. A slice thickness of 1mm means just what it says, each slice was 1mm thick. The specimen was scanned in a garnet silt wedge to help filter out any low energy X-rays and produce a well-calibrated, clear scan. This detector uses 512 detectors made of hand crafted cadmium tungsten crystals that are 0.25mm wide, 5mm high, and 5mm deep. 420kV is the potential of the scanner's electric field, and 4.8mA is its filament setting. The Pantak X-ray source is one of two ways that X-rays can be created with the CT Scanner. The image dimensions tell us that the original images are 512X512 pixels.
For the Herrerasaurus skull which was scanned by Richard Ketcham on 26 February 1998, the most important parameters and information used were: Image dimensions- 512X512 pixels Pantak X-ray source- 420kV, 4.8mA X-ray detector- P250D garnet silt wedge slice thickness- 1mm, interslice spacing- 0.8mm field of reconstruction- 151mm. For each specimen, different parameters and settings are needed to give the desired results.
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